DETOXIFICATION OF AFLATOXIN B-1 BY ENZYMES ISOLATED FROM ARMILLARIELLA-TABESCENS

Authors
LIU DL YAO DS LIANG R MA L CHENG WQ GU LQ
Citation
Dl. Liu et al., DETOXIFICATION OF AFLATOXIN B-1 BY ENZYMES ISOLATED FROM ARMILLARIELLA-TABESCENS, Food and chemical toxicology, 36(7), 1998, pp. 563
Citations number
15
Categorie Soggetti
Toxicology,"Food Science & Tenology
Journal title
Food and chemical toxicology → ACNP
ISSN journal
02786915
Volume
36
Issue
7
Year of publication
1998
Database
ISI
SICI code
0278-6915(1998)36:7<563:DOABBE>2.0.ZU;2-#
Abstract
Detoxification of aflatoxin B-1 (AFB(1)) by Armillariella tabescens mu ltienzyme, which was isolated from mycelium pellets of A. tabescens, w as confirmed by thin-layer chromatography (TLC) and rat assay. The res ults of toxicology and pathology studies showed that toxicity of AFB(1 ) was minimized after treatment with A. tabescens multienzyme. The res ult of the Ames test indicated that the mutagenic activity of multienz yme-treated AFB(1) was greatly reduced (or inactivated) compared with that of untreated controls. TLC determinations showed that AFB(1) at a n initial concentration of 16 mu M was completely detoxified (100%) by the fungal multienzyme. The infrared spectrum suggests that the multi enzyme is responsible for opening the difuran ring of AFB(1). (C) 1998 Elsevier Science Lid. All rights reserved.