DEVELOPMENT OF MONOCLONAL-ANTIBODY SANDWICH-ELISA FOR DETERMINATION OF BEER FOAM-ACTIVE PROTEINS

The development of a method to determine foam-active and haze-active p roteins by enzyme-linked immunosorbent assays (ELISA) based on polyclo nal antibodies was previously reported. Several different monoclonal a ntibodies against foam-active proteins in beer were developed. These m onoclonal antibodies have enabled us to develop a sandwich-ELISA for t he detection of foam-active proteins. Compared with our former ELISA, this new system showed the following advantages: The detection limits for the foam-active protein determined with the new ELISA system were greatly improved, and the contents of foam-active protein quantified b y new ELISA system were highly correlated with the foam adhesion on be er. This system was applied to the evaluation of foam-active proteins during the brewing process.