Jg. Clark et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF TETRAHYDRO-ISO-ALPHA-ACIDS IN BEER, Journal of the American Society of Brewing Chemists, 56(2), 1998, pp. 76-79
An improved method for the measurement of tetrahydro-iso-alpha-acids a
nd iso-alpha-acids in beer is presented. The method is based upon the
HPLC separation of hop acids on a C8 reverse-phase column using gradie
nt elution with a citrate buffer at a pH of 7.0. The method is suitabl
e for measurement of small additions of tetrahydro-iso-alpha-acids for
foam improvement as well as for the determination of trace amounts of
iso-alpha-acids in a beer designed to be light stable.