E. Lucker et al., PROCEDURES FOR THE DETECTION OF UNWANTED INGREDIENTS IN MEAT-PRODUCTSWITH REGARD TO BOVINE SPONGIFORM ENCEPHALOPATHY (BSE), Die Fleischwirtschaft, 78(8), 1998, pp. 896-898
A procedure is presented which facillitates the highly specific detect
ion of central nevrous system in meat products. This procedure employs
poly- or mono-clonal antibodies against the neuron specific enolase a
fter fat and protein extraction, SDS gel electrophoresis and western b
lot. This, the addition of central nervous tissue in meat products can
be detected with high specificity. So far, contents of bovine brain a
s low as 0,25% were detected in cooked sausages of the frankfurter and
liverwurst type.