C-13 NUCLEAR-MAGNETIC-RESONANCE CHARACTERIZATION OF THE REACTION-PRODUCTS OF LAMB PREGASTRIC LIPASE-CATALYZED HYDROLYSIS OF TRIBUTYRYLGLYCEROL

Lamb pregastric lipase (LPGL), extracted from the tongue root and epig lottis of suckling lamb, has been used to catalyze the hydrolysis of e mulsified tributyrylglycerol. Reactions were generally carried out at 35 degrees C and initial pH 7.0. The speciation of the products of the reaction has been examined by C-13 nuclear magnetic resonance spectro scopy. Varying rates of reaction were produced by increasing the amoun t of enzyme added relative to the amount of lipid. A system rate param eter, Delta pH/Delta t, which is the change in pH (Delta pH) from time zero to the time when the sample was removed (Delta t), has been deve loped. 1,2(2,3)-Dibutyrylglycerol and 2-monobutyrylglyceroI have been identified as products of LPGL-catalyzed hydrolysis, while 1,3-dibutyr ylglycerol and 1 (3)-monobutyrylglycerol are products of uncatalyzed a cyl transfer reactions.