ISOLATION OF INTACT TYPE-V COLLAGEN FROM FISH INTRAMUSCULAR CONNECTIVE-TISSUE

Authors
SATO K OHASHI C MURAKI M ITSUDA H YOKOYAMA Y KANAMORI M OHTSUKI K KAWABATA M
Citation
K. Sato et al., ISOLATION OF INTACT TYPE-V COLLAGEN FROM FISH INTRAMUSCULAR CONNECTIVE-TISSUE, Journal of food biochemistry, 22(3), 1998, pp. 213-225
Citations number
27
Categorie Soggetti
Biology,"Food Science & Tenology","Nutrition & Dietetics
Journal title
Journal of food biochemistry → ACNP
ISSN journal
01458884
Volume
22
Issue
3
Year of publication
1998
Pages
213 - 225
Database
ISI
SICI code
0145-8884(1998)22:3<213:IOITCF>2.0.ZU;2-L
Abstract
A purification method for intact type V collagen from fish intramuscul ar connective tissue war; developed. Collagens were extracted with 0.5 M acetic acid from fish intramuscular connective tissue, Intact type V collagen could not be isolated by conventional salt fractionation wh ich has been used for isolation of pepsinized collagens. isolation was achieved by ion-exchange chromatography in the presence of 5 M urea. This is the first report on isolation of intact type V collagen from m uscle tissue.