DEVELOPMENT OF THE MOUSE PASSIVE ABDOMINAL-WALL ANAPHYLAXIS (PAA) METHOD AND APPLICATION OF THE METHOD TO SEARCH FOR ANTIALLERGIC EFFECT OFFOODS

Food allergy, which generally belongs to the category of Type I hypers ensitivity reaction, is a serious health problem. Previously, we repor ted two types of mouse anaphylactic models, one using the abdominal wa ll as a site for both induction and estimation (AW method), and the ot her estimating the hypotension in mice sensitized passively with mouse monoclonal anti-DNP IgE. In this paper, we propose a passive abdomina l wall anaphylaxis (PAA) method for use in screening for anti-allergic substances. Normal mice were intraperitoneally sensitized with mouse monoclonal anti-DNP IgE (5 mu g/mouse) and 24 hours later, challenged with dinitrophenyl human serum albumin(10 mu g/50 mu L/site) on the ab dominal wall. The PAA was estimated in terms of the increase of vascul ar permeability at the challenged abdominal wall site. The PAA was inh ibited by the oral preadministration of diphenhydramine in a dose-depe ndent manner (PAA inhibition, PAAI). The anti-allergic activities of s uch well-known anti-allergic foods as oolong tea, garlic, and leek wer e confirmed by the PAAI test. Therefore, the PAA method appears to be applicable for the screening of anti-allergic substances in foods, sim ply, rapidly, and with high sensitivity.