Enzymatic hydrolysis of lactose is accompanied by galactosyl transfer
to other sugars, thereby producing oligosaccharides. These are hydroly
zed slowly, both in vitro and in vivo. They can be thought of as low m
olecular weight, non-viscous, water-soluble, dietary fibre. They are c
onsidered to be physiologically functional foods which promote the gro
wth of bifidobacteria in the colon and a wide variety of health benefi
ts has been claimed in connection with this effect. This article revie
ws the mechanism of oligosaccharide formation, and then discusses the
amount and nature of the products as well as the factors which influen
ce them. The appearance and disappearance of oligosaccharides is explo
red through consideration of the kinetics of tranferase activity. The
consequences of oligosaccharide formation for dairy processing, food a
nalysis, nutrition and health are then briefly discussed. (C) 1998 Els
evier Science Ltd. All rights reserved.