FORMATION OF MALONALDEHYDE IN THE PRESENCE OF PROBUCOL, AN ANTI-ATHEROSCLEROSIS DRUG

Formation and inhibition of malonaldehyde (MA) from blood plasma lipid s oxidized by Fenton's reagent in the absence or presence of probucol sopropylidenedithio)bis(2,6-di-tert-butyl-phenol)] and L-ascorbic acid were investigated. The amount of IMA formed was quantitatively analys ed by gas chromatography. L-Ascorbic acid inhibited MA formation by ab out 30% at the level of 4.0 mu mol, but the amount of MA formed was in creased by the presence of probucol. When 3.0 mu mol oxidized probucol was hydrolysed at pH 1, 3 and 5, 2616.5 nmol, 287.5 nmol and 103.9 nm ol MA were recovered, respectively. This is the first report of quanti tative analysis of MA formed from probucol on oxidation. (C) 1998 Publ ished by Elsevier Science Ltd. All rights reserved.