SIMULTANEOUS DETERMINATION OF MONOSACCHARIDES AND OLIGOSACCHARIDES BYNORMAL-PHASE HPLC

A rapid and highly sensitive method has been developed for the simulta neous determination of reducing monosaccharides and oligosaccharides b y normal-phase HPLC with fluorescence detection. The technique of line ar gradient elution on a TSKgel Amide-80 column was more suitable for saccharides separation. The post-column reaction was optimized for flu orometric detection. Under optimum conditions, the detection limits we re 0.3 similar to 15 ng for the reducing saccharides investigated. The calibration curves were approximately linear in the range of 2,000 si milar to 12.5 pmol for glucose, cellotriose, and cellopentaose. The co efficient of variation for glucose was less than 1%. Application of th e method for food analysis was successful.