QUANTITATIVE-DETERMINATION OF PHOSPHOLIPIDS IN SUNFLOWER OIL

Phospholipids from sunflower oil samples were enriched by using solid- phase extraction (SPE) cartridges and subsequently separated and analy zed by high-performance liquid chromatography (HPLC) with an ultraviol et detector. The recovery of individual phospholipids at different tot al concentrations in model oils and the repeatability of the method we re investigated. The results demonstrated the utility of SPE-H PLC for quantitative analysis of phospholipids in sunflower oil and the effec tiveness for concentrating, separating, identifying, and quantitating phospholipids in samples with phosphatide contents as low as 0.1%. Sam ples of sunflower oil at different stages of processing were analyzed, and phospholipid profiles in hexane-extracted oil, hat-pressed oil, a nd water-degummed oils were compared.