USE OF AN AUTOBIOLUMINESCENT SALMONELLA-HADAR TO MONITOR THE EFFECTS OF ACID AND TEMPERATURE TREATMENTS ON CELL-SURVIVAL AND VIABILITY ON LACTIC ACID-TREATED POULTRY CARCASSES
Da. Bautista et al., USE OF AN AUTOBIOLUMINESCENT SALMONELLA-HADAR TO MONITOR THE EFFECTS OF ACID AND TEMPERATURE TREATMENTS ON CELL-SURVIVAL AND VIABILITY ON LACTIC ACID-TREATED POULTRY CARCASSES, Journal of food protection, 61(11), 1998, pp. 1439-1445
To determine the long-term effects of a lactic acid rinse on viability
and recovery of pathogens, Salmonella Hadar was isolated from poultry
and bioluminescent constructs obtained by transformation with the lux
(CDABE) gene cassette from Photobacterium phosphoreum. Results indica
ted that the transformed Salmonella Hadar lux was otherwise phenotypic
ally similar to the wild-type strain. Viability studies were performed
by measuring luminescence following lactic acid treatment of turkey b
reast and subsequent storage at -12, 0, 5, and 10 degrees C. The abili
ty of the S. Hadar lux strain to recover was determined by monitoring
light output after incubation at 22 degrees C for 10 h. The results sh
owed that metabolic activity was significantly (P < 0.05) affected by
lactic acid and by storage temperatures of - 12, 0, and 5 degrees C. T
he lowest recovery rate was observed after rinsing with lactic acid an
d storing at 5 degrees C. The study demonstrated that bacterial biolum
inescence is an effective way of monitoring in ''real time'' the abili
ty of bacteria to recover from stress.