A method using methanolic sulphuric acid as transmethylating reagent w
as developed for determining the fatty acid composition of lipids of o
ats. The method was optimised for reaction conditions and applied to t
he determination of the fatty acid composition of lipids of a number o
f varieties of Australian oats grown in several locations. Thirteen fa
tty acids were detected with oleic, linoleic and palmitic acids compri
sing more than 95% of the total fatty acids. Total lipid content of th
e oats was positively related to the proportion of stearic (r=0.32) an
d oleic (r=0.81) acids and negatively correlated with the proportion o
f palmitic (r= -0.64), linoleic (r= -0.39) and linolenic (r= -0.65) ac
ids. Significant positive correlations were found between total lipid
content and absolute content of the major fatty acids (r=0.67 similar
to 0.98), except for linolenic acid (r=0.12). Environment had signific
ant effects on fatty acid composition, but variety was the controlling
factor. The broad sense heritability estimated from individual plot r
anged from 69 to 73% and that from the average of three replications a
nd eight locations ranged from 94 to 98% for the major fatty acids. It
is possible to improve fatty acid composition of oats by breeding pro
cedures. (C) 1998 Academic Press.