ISOLATION OF FLAVOPROTEIN FROM CHICKEN EGG-WHITE BY A SINGLE-STEP DEAE ION-EXCHANGE CHROMATOGRAPHIC PROCEDURE

Authors
RAO PF LIU ST WEI Z LI JC CHEN RM CHEN GR ZHENG YQ
Citation
Pf. Rao et al., ISOLATION OF FLAVOPROTEIN FROM CHICKEN EGG-WHITE BY A SINGLE-STEP DEAE ION-EXCHANGE CHROMATOGRAPHIC PROCEDURE, Journal of food biochemistry, 20(6), 1997, pp. 473-479
Citations number
10
Categorie Soggetti
Biology,"Nutrition & Dietetics
Journal title
Journal of food biochemistry → ACNP
ISSN journal
01458884
Volume
20
Issue
6
Year of publication
1997
Pages
473 - 479
Database
ISI
SICI code
0145-8884(1997)20:6<473:IOFFCE>2.0.ZU;2-E
Abstract
A single-step method to isolate flavoprotein from chicken egg white is described. Egg white diluted 10 fold with 50 mM sodium phosphate buff er, pH 6.5, was applied to a DEAE (Diethylaminoethyl cellulose)-Toyope arl 650 M column. The adsorbed proteins were fractionated into two pea ks by a linear salt gradient. The second peak war electrophoretically pure flavoprotein, with a total yield of about 75%.