DETECTION OF ANTIBIOTIC RESIDUES IN SLAUG HTER ANIMALS BY USING AN IMPEDIMETRIC METHOD

Microbiological inhibition tests like the German Three-Plates-Test (TP T) or the New Netherland-Kidney-Test (NNKT) are requiring a relatively large amount of time until test results are available. Moreover they do not seem to be appropriate for automation efforts. Using an impedim etric method could lead to certain improvements. In trials by using a Malthus Growth Analyzer, Bacillus stearothermophilus was found to be t he most appropriate test organism. After investigations in order to fi nd the appropriate framework, the sensitivity to detect certain antibi otics, i.e. Erythromycin, Gentamicin, Streptomycin, Chlortetracyclin, Oxytetracyclin, Penicillin G, Amoxicillin, Chloramphenicol and Polymyx in B, were examined and compared with the sensitivity of TPT and NNKT. Reliable results were available after an incubation period of 3.5 h. The sensitivity were within the maximum residue limits (MRLs) which we re given by the European Community and seemed to be almost equivalent to those of the TPT. When using the impedimetric method, we suggest th e use of meat liquid which can be gained by centrifugation.