IDENTIFICATION OF CYCLOPENTENYL FATTY-ACIDS BY H-1 AND C-13 NUCLEAR-MAGNETIC-RESONANCE

Gorlic, chaulmoogric and hydnocarpic fatty acids, specific to the seed oil of the genus Hydnocarpus sp. (Flacourtiaceae), are determined onl y with difficulty by gas chromatography. These fatty acids were isolat ed in their methyl ester form by a combination of different chromatogr aphic techniques (thin-layer chromatography/Ag+ and high-pressure liqu id chromatography). The proton and carbon nuclear magnetic resonance a nalysis of these fatty acid methyl esters showed some characteristic s ignals of the cyclopentenyl ring. The presence of these signals in the proton and/or carbon nuclear magnetic resonance spectrum of an oil th us will allow us to confirm the presence of these cyclopentenyl fatty acids in lipids.