DETERMINATION OF PEFLOXACIN IN SERUM AND PHARMACEUTICAL FORMS BY DERIVATIVE SPECTROPHOTOMETRY

A method for the direct determination of pefloxacin in serum and pharm aceutical forms (tablets and ampoules) has been developed, based on th e use of second-order derivative ultraviolet spectra. Spectrophotometr ic assay of pefloxacin in tablets and ampoules was carried out in 0.1 mol/L NaOH, while in serum it was performed in 0.1 mol/L NaOH with the addition of sodium dodecylsulfate, in 337-347 nm wavelength range. Li near calibration curves were obtained in the concentration ranges 2-30 mu g/mL pefloxacin for tablets and ampoules and 0.12-5 mu g/mL for se rum samples. Relative error of determination, as criterion for accurac y, was less than 1%, while the precision was better than 4 ng/ml. The minimum detectable concentration of pefloxacin in serum was 15 ng/mL.