NUMERICAL ACQUISITION OF MULTIPLE IMMUNOFLUORESCENCE LABELING

Tools used in numerical acquisition of epi-fluorescence images are eva luated. Using multiple-band filter sets, the intensities of blue and r ed emitting fluorochromes are reduced. Color images may be reconstruct ed from monochrome images. Nevertheless, they may have X/Y and Z shift ed and need to be realigned before being superimposed. Confocal laser scanning microscope (CLSM) presents an advantage mainly for 3D microsc opy of thick specimens. Apart from camera performances, user interface is a key point in image acquisition.