In this paper, a central composite design has been applied to evaluate
, by means of empirical modelling of the data, the importance of dough
yield, NaCl, type of wheat flour, as well as fructose and citrate add
ition, on the growth and fermentative activity of mixed sourdough star
ters made by selected lactic acid bacteria and yeast. The dough yield
variable was important in increasing yeast and lactic acid bacteria gr
owth. The stimulating effect of NaCl on yeasts can be indirectly attri
buted to a reduction in competition with lactic acid bacteria for the
available sugar. The latter were in turn inhibited by NaCl above a cer
tain threshold level. Citrate and fructose modification can be used to
optimize the relative ratios of acetic and lactic acids. This prelimi
nary work considered neither microstructural rheological features of t
he dough, nor bread organoleptic properties. However, the results conf
irmed the key role of some ingredients in the interaction among the so
urdough starters and in the regulation of their metabolism. (C) 1997 A
cademic Press Limited.