ATOMIC-FORCE MICROSCOPY OF PLASMID DNA AND DNA-POLYMERASE

Authors
LI MQ HANSMA HG HONG GF YAO XW HANSMA PK
Citation
Mq. Li et al., ATOMIC-FORCE MICROSCOPY OF PLASMID DNA AND DNA-POLYMERASE, Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena, 12(3), 1994, pp. 1465-1469
Citations number
12
Categorie Soggetti
Physics, Applied
Journal title
Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena → ACNP
ISSN journal
10711023
Volume
12
Issue
3
Year of publication
1994
Pages
1465 - 1469
Database
ISI
SICI code
1071-1023(1994)12:3<1465:AMOPDA>2.0.ZU;2-F
Abstract
An improved sample preparation procedure and optimized atomic force mi croscope (AFM) imaging conditions that makes it possible to obtain rep roducible nanometer resolution AFM images of plasmid DNA with nanogram s of the sample is presented. The Bst DNA polymerase I is a special ki nd of DNA polymerase that can keep its function even at high temperatu re (approximately 65-degrees-C), but little is known about its surface structure. The AFM images of the Bst DNA polymerase I show its specia l surface structure.