SCANNING FORCE MICROSCOPY OF ESCHERICHIA-COLI OMPF PORIN IN BUFFER SOLUTION

Authors
SCHABERT FA HOH JH KARRASCH S HEFTI A ENGEL A
Citation
Fa. Schabert et al., SCANNING FORCE MICROSCOPY OF ESCHERICHIA-COLI OMPF PORIN IN BUFFER SOLUTION, Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena, 12(3), 1994, pp. 1504-1507
Citations number
15
Categorie Soggetti
Physics, Applied
Journal title
Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena → ACNP
ISSN journal
10711023
Volume
12
Issue
3
Year of publication
1994
Pages
1504 - 1507
Database
ISI
SICI code
1071-1023(1994)12:3<1504:SFMOEO>2.0.ZU;2-Z
Abstract
The extracellular and periplasmic surface topography of the E. coli ou ter membrane protein OmpF reconstituted into 2D crystals in the presen ce of phospholipids were investigated with a scanning force microscope in buffer solution. Topographs exhibiting 2 nm lateral resolution are in good agreement with data from electron microscopy and x-ray crysta llography, and indicate the exciting possibility to monitor structural changes of ''live systems'' at molecular resolution by scanning force microscopy.