Evolution of free amino acids (FAA) during ripening (10-300 days) of f
ive batches of Mahon cheese was studied; three batches were made from
raw milk and two from pasteurized milk. Major FAA were GLU, VAL, LEU,
LYS and PHE (43-67% of total FAA). Relative THR, SER, GLU and GLY cont
ents significantly increased during ripening whereas LEU, PHE and ORN
decreased. GLU, ILE, SER, THR, GLY and PHE varied during ripening acco
rding to a zero order reaction (r(2) > 0.99). Principal component anal
ysis showed that the first principal component could be considered rep
resentative of the ripening time. The most ripened cheeses showed high
GLU, GLY, SER and THR contents and the less ripened cheeses presented
high ORN, LEU, PHE and ALA contents. The second component distinguish
ed between cheeses made with pasteurized milk (with high ASN and GLN c
ontents) and raw milk. Indigenous microflora of raw milk showed a stro
ng influence on the proteolytic activity in Mahon cheese. (C) 1997 Els
evier Science Ltd.