THERMOSPRAY-LMCS-INVESTIGATIONS FOR PROAN THOCYANIDINES AND OTHER POLYPHENOLS IN MALT, BEER AND HOP

Thermospray-LCMS-analyses of polyphenols in malt, beer and hop are des cribed. After extraction and polyamide-clean-up it is possible to dete ct monomeric catechins and proanthocyanidins in different fractions as [M+H](+)-ions by buffer ionisation. Using the discharge electrode phe nolic acids are identified in malt and beer as [M-H](-)-ions. Besides quinic acid esters and flavonol O-glycosides, showing significant frag mentation in the tsp-spectrums like the proanthocyanidins, are additio nally detected in hop. Further more the HPLC method was used to estima te the quantitative value of proanthocyanidins in malt.